Shelton · International journal of cancer 2010 · controlled animal study · n=?

Glutamine targeting inhibits systemic metastasis in the VM-M3 murine tumor model.

Level 5 - mechanism / opinion, no new human data

Preclinical animal study with no human data.

PubMed 20473919 · doi:10.1002/ijc.25431 · record verified 2026-08-26

What was done

Researchers evaluated the antimetastatic effects of 6-diazo-5-oxo-L-norleucine (DON), a glutamine analog, using the VM-M3 murine model of systemic metastasis. Primary tumor growth and metastatic spread to the liver, lung, and kidney were assessed using bioluminescence imaging and histology in DON-treated mice compared to untreated controls.

What was found

Primary tumor growth was approximately 20-fold lower in DON-treated mice compared to untreated controls. Treatment with DON also inhibited metastasis to the liver, lung, and kidney.

Why it matters

This paper provides preclinical proof of concept that targeting glutamine metabolism can suppress both primary tumor progression and systemic metastatic dissemination in a mouse model.

Limits

The study is limited to a single preclinical mouse model (VM-M3) and cannot be directly translated to human clinical efficacy. The abstract reports no sample sizes (n), dosing regimens, toxicity outcomes, survival metrics, or exact quantitative values/confidence intervals for metastatic burden.

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