Balban · Cell reports. Medicine 2023 · randomized controlled trial · n=?

Brief structured respiration practices enhance mood and reduce physiological arousal.

Level 2 - randomized trial

Individual randomized controlled trial comparing three breathwork protocols to mindfulness meditation.

PubMed 36630953 · doi:10.1016/j.xcrm.2022.100895 · record verified 2026-08-26

What was done

A remote randomized controlled trial (NCT05304000) evaluated 1 month of daily 5-minute interventions across four groups: three structured breathwork protocols—cyclic sighing (prolonged exhalations), box breathing (equal inhalations, retentions, and exhalations), and cyclic hyperventilation with retention—compared with daily 5-minute mindfulness meditation. Primary outcomes included changes in mood, anxiety, and physiological arousal markers (respiratory rate, heart rate, and heart rate variability) analyzed with a mixed-effects model.

What was found

Breathwork, particularly cyclic sighing, demonstrated a statistically significant advantage over mindfulness meditation for mood improvement (p < 0.05) and respiratory rate reduction (p < 0.05). The abstract does not report participant counts, numerical effect sizes, confidence intervals, or specific statistical findings for heart rate, heart rate variability, or anxiety.

Why it matters

This suggests that structured, brief daily breathwork—specifically cyclic sighing—may reduce physiological arousal and enhance mood more rapidly or effectively than standard mindfulness meditation.

Limits

The abstract does not provide the sample size (n), participant demographics, baseline stress levels, adherence rates, or raw numerical effect sizes. Long-term retention of benefits beyond the 1-month intervention was not assessed.

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