Chen · American journal of human genetics 1999 · case-control study · n=885

Interaction between the functional polymorphisms of the alcohol-metabolism genes in protection against alcoholism.

Cited 475 times in the scientific literature.

Level 4 - case-series / case-control

Case-control genetic association study

PubMed 10441588 · doi:10.1086/302540 · record verified 2026-08-26

What was done

A case-control genetic association study genotyped 340 alcoholic and 545 control Han Chinese individuals living in Taiwan at three functional polymorphic loci: ADH2, ADH3, and ALDH2. Multiple logistic regression analysis was performed to test for independent effects and interactions among combinatorial genotypes on the risk of alcoholism.

What was found

ALDH2*2 homozygosity was entirely protective against alcoholism regardless of ADH2 genotype (0 individuals found among the 340 alcoholic cases). When controlling for ALDH2*2, ADH3 variation showed no significant effect due to linkage disequilibrium with ADH2*2. Compared to the reference ADH2*1/*1 and ALDH2*1/*1 genotype, individuals with one or two copies of ADH2*2 plus a single ALDH2*2 copy had the lowest risk of alcoholism (ORs 0.04-0.05). Risk for the ADH2*2/*2-ALDH2*1/*1 genotype was approximately half that of ADH2*1/*2-ALDH2*1/*1.

Why it matters

The findings demonstrate that ADH2*2 and ALDH2*2 provide independent protective effects against alcoholism, while clarifying that ADH3 does not independently contribute.

Limits

The study is restricted to a single ethnic group (Han Chinese in Taiwan). As a case-control design, it is susceptible to selection bias, and the abstract does not report confidence intervals, exact p-values, or clinical diagnostic criteria for alcoholism.

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