Lin · Cell 1999 · Animal genetic mapping and positional cloning study · n=?

The sleep disorder canine narcolepsy is caused by a mutation in the hypocretin (orexin) receptor 2 gene.

Cited 2631 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal genetic study (preclinical bench/animal research)

PubMed 10458611 · doi:10.1016/s0092-8674(00)81965-0 · record verified 2026-08-26

What was done

Positional cloning was used to identify the autosomal recessive gene mutation responsible for narcolepsy in a canine model characterized by daytime sleepiness, cataplexy, and rapid eye movement (REM) sleep transitions.

What was found

The abstract reports no numerical data. It established that canine narcolepsy is caused by disruption of the hypocretin (orexin) receptor 2 gene (*Hcrtr2*).

Why it matters

This study identified hypocretin neuropeptides as essential regulators of sleep architecture, uncovering a major pathway underlying narcolepsy and opening new therapeutic targets.

Limits

The investigation was conducted exclusively in a canine model, and the abstract provides no sample size or specific genetic sequence metrics. Findings in genetic canine narcolepsy do not fully capture human narcolepsy, which is typically sporadic and involves autoimmune loss of hypocretin-producing neurons rather than receptor mutations.

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