Yu · Journal of neuroscience research 1999 · controlled animal experiment and in vitro study · n=?

Dietary restriction and 2-deoxyglucose administration reduce focal ischemic brain damage and improve behavioral outcome: evidence for a preconditioning mechanism.

Cited 361 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal and in vitro laboratory study

PubMed 10467254 · record verified 2026-08-30

What was done

Adult rats were maintained on a dietary restriction regimen or administered 2-deoxyglucose while fed ad libitum, and then subjected to a middle cerebral artery occlusion-reperfusion model of focal stroke. Researchers assessed ischemic brain damage, behavioral outcomes, and striatal heat-shock protein 70 levels. In parallel, cultured hippocampal neurons were treated with 2-deoxyglucose and exposed to chemical hypoxia to assess direct neuroprotection and cellular heat-shock protein 70 induction.

What was found

The abstract reports no numerical values, sample sizes, or effect estimates. Qualitatively, dietary restriction and 2-deoxyglucose administration each reduced brain damage and improved behavioral outcomes after middle cerebral artery occlusion-reperfusion in rats. In vitro, 2-deoxyglucose protected cultured hippocampal neurons against chemical hypoxia. Both interventions in vivo, as well as 2-deoxyglucose treatment in vitro, increased heat-shock protein 70 levels.

Why it matters

The study indicates that mild metabolic stress from caloric restriction or glucose metabolism inhibition can trigger preconditioning stress responses that protect brain tissue from ischemic injury.

Limits

The experiments were performed exclusively in rodent and in vitro models, which cannot establish efficacy or safety in humans. The abstract provides no quantitative data, sample sizes, specific dietary restriction regimens, dosing details, or long-term functional recovery measures.

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