Kushad · Journal of agricultural and food chemistry 1999 · comparative phytochemical analysis across plant accessions · n=65 accessions

Variation of glucosinolates in vegetable crops of Brassica oleracea.

Cited 594 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Level 5 by design analogy; agricultural and laboratory phytochemical study without human data.

PubMed 10564014 · doi:10.1021/jf980985s · record verified 2026-08-30

What was done

Researchers measured glucosinolate profiles and concentrations across 65 accessions representing five crop groups of *Brassica oleracea* (50 broccoli, 4 Brussels sprouts, 6 cabbage, 3 cauliflower, and 2 kale) grown under uniform cultural conditions.

What was found

Glucosinolate concentrations and types varied significantly across and within groups. In broccoli, the predominant glucosinolates were glucoraphanin (4-methylsulfinylbutyl glucosinolate), gluconapin (3-butenyl glucosinolate), and glucobrassicin (3-indolylmethyl glucosinolate), with glucoraphanin ranging from 0.8 µmol/g dry weight (DW) in EV6-1 to 21.7 µmol/g DW in Brigadier. In Brussels sprouts, cabbage, cauliflower, and kale, the predominant glucosinolates were sinigrin (8.9, 7.8, 9.3, and 10.4 µmol/g DW, respectively) and glucobrassicin (3.2, 0.9, 1.3, and 1.2 µmol/g DW, respectively). Brussels sprouts also contained 6.9 µmol/g DW of gluconapin.

Why it matters

Demonstrating wide genetic variation in glucosinolate content, especially glucoraphanin in broccoli, identifies specific cultivars suitable for breeding programs aimed at enhancing dietary bioactive compounds.

Limits

This study evaluated only raw plant chemistry under uniform laboratory/agricultural conditions without evaluating human bioavailability, metabolism, preparation/cooking effects, or clinical health endpoints. Accession numbers were heavily skewed toward broccoli (50 accessions) relative to kale (2), cauliflower (3), Brussels sprouts (4), and cabbage (6).

Cited by