Mihic · Medicine and science in sports and exercise 2000 · randomized double-blind controlled trial · n=30

Acute creatine loading increases fat-free mass, but does not affect blood pressure, plasma creatinine, or CK activity in men and women.

Cited 201 times in the scientific literature.

Level 2 - randomized trial

Individual randomized, double-blind controlled trial

PubMed 10694109 · doi:10.1097/00005768-200002000-00007 · record verified 2026-08-29

What was done

Thirty participants (15 men and 15 women) were evaluated in a randomized, double-blind study examining the effects of acute creatine monohydrate loading (20 g per day for 5 days) versus placebo. Measured outcomes included systolic, diastolic, and mean blood pressure, plasma creatinine, estimated creatinine clearance, plasma creatine kinase (CK) activity, body composition, and ischemic isometric handgrip strength.

What was found

Creatine loading significantly increased fat-free mass and total body mass compared to placebo (P < 0.05), with greater gains in men than women, while body fat did not change. No significant differences were observed for blood pressure, plasma creatinine, estimated creatinine clearance, plasma CK activity, or handgrip strength (P > 0.05). No exact numerical values or effect sizes were reported in the abstract.

Why it matters

This paper demonstrates that short-term high-dose creatine supplementation enhances lean mass without acutely impairing blood pressure, renal function markers, or muscle damage indicators in healthy men and women.

Limits

The sample size was small (30 participants total across both sexes) and the intervention was very short (5 days). The abstract omits numerical values, baseline participant characteristics, and measures of longer-term safety or varied athletic performance outcomes.

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