Zeitzer · The Journal of physiology 2000 · Controlled human laboratory dose-response trial · n=23

Sensitivity of the human circadian pacemaker to nocturnal light: melatonin phase resetting and suppression.

Cited 1183 times in the scientific literature.

Level 3 - non-randomized controlled study

Controlled non-randomized human experimental laboratory study

PubMed 10922269 · doi:10.1111/j.1469-7793.2000.00695.x · record verified 2026-08-27

What was done

Twenty-three healthy young male and female volunteers took part in a 9-day laboratory protocol assessing circadian pacemaker sensitivity to evening light. Participants received a single 6.5-hour experimental light exposure during the early biological night across a range of illuminance levels. Endogenous circadian phase changes in the melatonin rhythm and acute plasma melatonin suppression were measured.

What was found

Both phase-delay resetting and acute melatonin suppression followed a non-linear logistic dose-response curve. Approximately half of the maximal phase-delaying response observed at ~9,000 lux was achieved with dim room light of approximately 100 lux (just over 1% of bright light intensity). The same proportion applied to acute plasma melatonin suppression.

Why it matters

Ordinary dim-to-moderate indoor room light in the evening can substantially shift the human circadian clock and suppress melatonin, meaning daily lighting habits strongly influence circadian entrainment.

Limits

Small sample size (n = 23) restricted to healthy young adults. Exact phase shift magnitudes (in hours) and statistical confidence intervals were omitted from the abstract. A continuous 6.5-hour light exposure does not fully replicate typical real-world intermittent light exposure.

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