Wortsman · The American journal of clinical nutrition 2000 · matched non-randomized comparative study · n=?

Decreased bioavailability of vitamin D in obesity.

Cited 3198 times in the scientific literature.

Level 3 - non-randomized controlled study

Non-randomized matched comparative physiological study

PubMed 10966885 · doi:10.1093/ajcn/72.3.690 · record verified 2026-08-30

What was done

Healthy, white obese adults (BMI ≥ 30 kg/m²) and matched lean controls (BMI ≤ 25 kg/m²) were evaluated under two experimental conditions: whole-body ultraviolet radiation or an oral dose of 50,000 IU (1.25 mg) vitamin D₂. Researchers measured basal 25-hydroxyvitamin D, parathyroid hormone, blood vitamin D₃ 24 hours post-irradiation, skin 7-dehydrocholesterol content, in vitro previtamin D₃ photoproduction, and peak serum vitamin D₂ concentrations after oral administration.

What was found

Obese individuals had significantly lower baseline 25-hydroxyvitamin D and higher parathyroid hormone levels than matched controls. The 24-hour incremental rise in serum vitamin D₃ after whole-body irradiation was 57% lower in obese participants. Skin 7-dehydrocholesterol levels and in vitro conversion to previtamin D₃ did not differ significantly between groups. BMI was inversely correlated with serum vitamin D₃ after irradiation (r = -0.55, P = 0.003) and with peak serum vitamin D₂ after oral intake (r = -0.56, P = 0.007).

Why it matters

This study shows that obesity-related vitamin D deficiency stems from decreased systemic bioavailability and adipose sequestration rather than impaired cutaneous synthesis. This provides a mechanistic basis for why individuals with obesity often require higher vitamin D doses.

Limits

The abstract does not disclose the sample size. The cohort was restricted entirely to white participants, limiting direct generalizability to other skin phototypes. Direct measurement of adipose tissue vitamin D storage was not reported in the abstract.

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