Legrain · The Journal of clinical endocrinology and metabolism 2000 · randomized incomplete block trial · n=24

Dehydroepiandrosterone replacement administration: pharmacokinetic and pharmacodynamic studies in healthy elderly subjects.

Cited 100 times in the scientific literature.

Level 2 - randomized trial

Randomized incomplete block pharmacokinetic trial in humans

PubMed 10999810 · doi:10.1210/jcem.85.9.6805 · record verified 2026-08-29

What was done

Twenty-four healthy older men and women (mean age 67.8 ± 4.3 years) received daily oral DHEA (25 mg or 50 mg) or placebo for 8 days in a balanced incomplete block design. Blood was sampled nine times on day 1 and day 8 to evaluate the pharmacokinetics and concentrations of DHEA, DHEAS, testosterone, 5α-androstan-3α,17β-diol glucuronide, estradiol, and estrone.

What was found

Oral DHEA administration restored low baseline DHEA and DHEAS to youthful levels. Blood DHEA displayed an apparent terminal half-life of more than 20 hours, comparable to blood DHEAS, which the authors attributed to back-hydrolysis of formed DHEAS. Metabolic conversion of DHEAS to DHEA was significantly greater in women than in men. No accumulation of steroids was observed, and no concerning transformation to androgens or estrogens was recorded, with only a limited increase in estradiol observed in older women. The abstract did not report exact numerical pharmacokinetic values or confidence intervals.

Why it matters

This study demonstrates that oral DHEA provides sustained circulating steroid levels via back-hydrolysis of a DHEAS reservoir, supporting the feasibility and safety of a 50 mg daily dosing regimen for subsequent long-term clinical trials in elderly populations.

Limits

The study had a very small sample size (n = 24) and a very short treatment duration (8 days), precluding any assessment of long-term safety, sustained endocrine effects, or clinical efficacy outcomes. Specific numerical values (such as clearance, AUC, or exact hormone levels) were omitted from the abstract.

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