Sack · The New England journal of medicine 2000 · placebo-controlled crossover trial · n=7

Entrainment of free-running circadian rhythms by melatonin in blind people.

Cited 495 times in the scientific literature.

Level 2 - randomized trial

Placebo-controlled crossover clinical trial

PubMed 11027741 · doi:10.1056/NEJM200010123431503 · record verified 2026-08-29

What was done

Seven totally blind individuals with free-running circadian rhythms participated in a crossover trial comparing 10 mg of daily melatonin against placebo, taken one hour before preferred bedtime for three to nine weeks per phase. Circadian phase was tracked using endogenous melatonin production timing, and sleep parameters were recorded with polysomnography. Three subjects also underwent a stepwise dose-reduction protocol down to 0.5 mg daily over three months.

What was found

Baseline circadian cycles averaged 24.5 hours (range, 24.2 to 24.9) and were unaffected by placebo. During melatonin administration, rhythms entrained to a 24.0-hour cycle in 6 of 7 participants (P<0.001). Post-entrainment, time awake after sleep onset decreased significantly (P=0.05) and sleep efficiency showed a non-significant trend toward improvement (P=0.06). Entrainment persisted at 0.5 mg daily in all 3 subjects tested during dose tapering.

Why it matters

It demonstrates that exogenous melatonin can act as an effective non-photic time cue to synchronize free-running endogenous clocks to the 24-hour day in people without light perception.

Limits

The sample size is very small (n=7 total, n=3 for dose de-escalation). The abstract does not report whether crossover allocation was randomized or double-blinded, sleep efficiency gains did not reach conventional statistical significance (P=0.06), and long-term clinical outcomes like daytime alertness were not quantified.

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