Macrides · Science (New York, N.Y.) 1975 · controlled animal laboratory experiment · n=?

Strange females increase plasma testosterone levels in male mice.

Cited 184 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Non-human animal study

PubMed 1162363 · doi:10.1126/science.1162363 · record verified 2026-08-26

What was done

Male house mice paired with a normal female for 1 week were evaluated for plasma testosterone changes under different social manipulations: remaining with the familiar female, comparison to all-male housing, replacement of the resident female with a novel (strange) female, replacement with a novel male, or initial pairing following isolation. Conditions were evaluated at 30 to 60 minutes post-exposure, including under housing allowing ambient odor exposure and accounting for whether copulation occurred.

What was found

Males paired with a female for 1 week had plasma testosterone levels comparable to males in all-male groups. Introducing a strange female in place of the resident female led to markedly elevated plasma testosterone levels at 30 to 60 minutes. This surge was similar in magnitude to that observed when isolated males were first paired with a female, did not require copulation, persisted in the presence of background male and female odors, and did not occur when the female was replaced with an unfamiliar male. The abstract reports directional patterns without exact numerical concentrations.

Why it matters

This study demonstrates a rapid and specific neuroendocrine response in male mammals triggered by novel social and sexual cues rather than physical copulation alone.

Limits

The abstract provides no sample sizes, variance metrics, or exact hormone concentrations. Findings are restricted to male house mice and cannot be directly generalized to other species or humans.

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