Rho · Epilepsia 2002 · Animal experimental study · n=?

Acetoacetate, acetone, and dibenzylamine (a contaminant in l-(+)-beta-hydroxybutyrate) exhibit direct anticonvulsant actions in vivo.

Cited 157 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal model testing direct pharmacological effects in mice

PubMed 11952765 · doi:10.1046/j.1528-1157.2002.47901.x · record verified 2026-08-30

What was done

Researchers evaluated the direct anticonvulsant effects of acetoacetate (ACA), acetone, and the D-(-) and L-(+) stereoisomers of beta-hydroxybutyrate (BHB) against sensory-evoked seizures in Frings audiogenic seizure-susceptible mice. Gas chromatography-mass spectrometry was used to analyze compound purity.

What was found

Acetoacetate and acetone showed anticonvulsant actions in this mouse model, whereas the physiological D-(-)-BHB isomer was not anticonvulsant. The anticonvulsant activity observed with L-(+)-BHB was found to be attributable to a chemical contaminant, dibenzylamine. The abstract reports no numerical values or effect sizes.

Why it matters

This study suggests that the seizure-protective effects of the ketogenic diet may be directly mediated by specific ketone bodies, namely acetoacetate and acetone, rather than D-(-)-beta-hydroxybutyrate.

Limits

The study was performed entirely in an audiogenic mouse model, which may not translate directly to human epilepsy. The abstract provides no sample sizes, dosages, statistical metrics, or quantitative results.

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