Anson · Proceedings of the National Academy of Sciences of the United States of America 2003 · Controlled animal experiment · n=?

Intermittent fasting dissociates beneficial effects of dietary restriction on glucose metabolism and neuronal resistance to injury from calorie intake.

Cited 737 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal research (preclinical mouse model)

PubMed 12724520 · doi:10.1073/pnas.1035720100 · record verified 2026-08-30

What was done

C57BL6 mice were maintained on an intermittent fasting (alternate-day fasting) regimen to evaluate its effects on overall food intake, body weight, glucose metabolism, and neuronal vulnerability. Serum glucose and insulin levels were assessed, along with brain neuronal resistance to excitotoxic stress.

What was found

The abstract reports no numerical values, sample sizes, or effect sizes. Qualitatively, intermittent fasting did not decrease overall food intake and maintained body weight. Intermittent fasting resulted in reduced serum glucose and insulin levels and increased resistance of brain neurons to excitotoxic stress, matching or exceeding effects typically attributed to caloric restriction.

Why it matters

This study demonstrates in a mouse model that the metabolic and neuroprotective benefits of dietary restriction can occur through meal timing and fasting intervals independently of net calorie reduction.

Limits

The study was conducted entirely in mice, preventing direct clinical extrapolation to humans. The abstract omits sample sizes, experimental durations, and exact numerical measurements for glucose, insulin, or neuronal survival.

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