Ouyang · Journal of clinical immunology 2003 · cross-sectional observational study · n=?

Large numbers of dysfunctional CD8+ T lymphocytes bearing receptors for a single dominant CMV epitope in the very old.

Cited 240 times in the scientific literature.

Level 4 - case-series / case-control

Cross-sectional observational comparison between young and elderly individuals without clinical intervention

PubMed 12959217 · doi:10.1023/a:1024580531705 · record verified 2026-08-29

What was done

Using MHC-peptide tetramer technology, the authors analyzed peripheral CD8+ T lymphocytes specific to a single cytomegalovirus (CMV) epitope, comparing very elderly individuals (>85 years) to younger controls. They assessed epitope-specific T-cell numbers, CD28 expression, and functional capacity measured by interferon-gamma (IFN-γ) secretion following antigenic stimulation.

What was found

The abstract reports no exact numbers, percentages, or effect sizes. It reports that very elderly individuals had markedly higher numbers of CD8+ T cells specific for a single CMV epitope. Compared to younger individuals, these tetramer-reactive CD8+ T cells in the elderly had a significantly lower percentage of CD28 expression and a significantly lower fraction secreting IFN-γ after specific stimulation.

Why it matters

This study provides cellular-level evidence that massive, dysfunctional clonal expansions of CMV-specific CD8+ T cells occur in extreme old age, which may constrict the naive T-cell repertoire available for other pathogens.

Limits

The abstract reports no sample sizes (n is unknown), no quantitative measurements, no p-values, and no details on participant health status or selection criteria. The cross-sectional design demonstrates phenotypic and functional differences but cannot prove a causal relationship with susceptibility to other infections.

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