Anderson · JAMA 2004 · randomized double-blind placebo-controlled trial · n=10739

Effects of conjugated equine estrogen in postmenopausal women with hysterectomy: the Women's Health Initiative randomized controlled trial.

Cited 4493 times in the scientific literature.

Level 2 - randomized trial

Individual randomized double-blind placebo-controlled trial

PubMed 15082697 · doi:10.1001/jama.291.14.1701 · record verified 2026-08-26

What was done

A multicenter, randomized, double-blind, placebo-controlled trial across 40 US clinical centers evaluated the effect of daily conjugated equine estrogen (CEE, 0.625 mg/d) versus placebo on chronic disease incidence in 10,739 postmenopausal women aged 50-79 years with prior hysterectomy (23% minority race/ethnicity). The primary efficacy outcome was coronary heart disease (nonfatal myocardial infarction or CHD death), the primary safety outcome was invasive breast cancer, and a global index tracked overall risks and benefits over an average follow-up of 6.8 years.

What was found

Compared with placebo, CEE showed no significant effect on coronary heart disease (HR 0.91, 95% CI 0.75-1.12; 376 cases) or invasive breast cancer (HR 0.77, 95% CI 0.59-1.01; 218 cases). CEE significantly increased risk of stroke (HR 1.39, 95% CI 1.10-1.77; 276 cases; absolute excess of 12 per 10,000 person-years) and total cardiovascular disease (HR 1.12, 95% CI 1.01-1.24). CEE significantly reduced risk of hip fracture (HR 0.61, 95% CI 0.41-0.91; 102 cases; absolute reduction of 6 per 10,000 person-years) and total fractures (HR 0.70, 95% CI 0.63-0.79). No significant differences were seen for pulmonary embolism (HR 1.34, 95% CI 0.87-2.06), colorectal cancer (HR 1.08, 95% CI 0.75-1.55), total cancer (HR 0.93, 95% CI 0.81-1.07), total mortality (HR 1.04, 95% CI 0.88-1.22), or the global index (HR 1.01, 95% CI 0.91-1.12).

Why it matters

This landmark trial demonstrated that estrogen-alone therapy does not reduce coronary heart disease and increases stroke risk, establishing that CEE should not be recommended for chronic disease prevention in postmenopausal women with prior hysterectomy.

Limits

The intervention phase was terminated early by the NIH. The findings are specific to oral conjugated equine estrogen (0.625 mg/d) in postmenopausal women with prior hysterectomy aged 50-79, and do not directly evaluate other formulations, lower doses, non-oral routes, or women with an intact uterus.

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