Apolipoproteins A-IV and A-V are acute-phase proteins in mouse HDL.
Level 5 - mechanism / opinion, no new human data
Animal model and in vitro bench experiment
PubMed 15306172 · doi:10.1016/j.atherosclerosis.2004.04.018
What was done
Mice were injected with endotoxin to model acute inflammation, and HDL was isolated. Protein composition changes were analyzed using two-dimensional gel electrophoresis and mass spectrometry. Hepatic mRNA levels of candidate apolipoproteins were measured in the mice, and interleukin-6 (IL-6) was applied to Hep3B cells to assess mRNA response in vitro.
What was found
The abstract reports no numerical values. Directionally, endotoxin injection in mice resulted in: - Increases in apo SAA, apo E, apo A-IV, and apo A-V protein on isolated HDL. - Decreases in apo A-I and apo A-II protein on HDL. - Increased hepatic mRNA expression of apo A-IV and apo A-V, and decreased hepatic mRNA expression of apo A-II. - In Hep3B cells, IL-6 increased apo A-IV and apo A-V mRNA levels.
Why it matters
This study identifies apo A-IV and apo A-V as positive acute-phase HDL proteins in mice, showing that hepatic upregulation alters HDL composition during inflammatory states.
Limits
The findings are limited to mouse models and cultured cell lines, which may not fully reflect human physiology. The abstract omits sample sizes, quantitative effect sizes, variance, and functional assessments of how these proteomic changes affect atherogenesis.
Cited by
- supports Cholesterol components can act as acute-phase reactants that change acutely in response to stress and inflammation.