Kuba · Nature medicine 2005 · Preclinical in vivo animal experiment · n=?

A crucial role of angiotensin converting enzyme 2 (ACE2) in SARS coronavirus-induced lung injury.

Cited 3650 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal model and preclinical mechanistic study

PubMed 16007097 · doi:10.1038/nm1267 · record verified 2026-08-30

What was done

Experimental in vivo mouse study evaluating the role of ACE2 as a SARS-CoV receptor during acute lung injury. Investigators tested the effects of SARS-CoV infection and SARS-CoV Spike protein on ACE2 expression levels and assessed whether administering the Spike protein exacerbated lung failure and whether blocking the renin-angiotensin pathway modified this injury.

What was found

The abstract reports directional findings without numerical values or effect sizes. SARS-CoV infection and SARS-CoV Spike protein reduced ACE2 expression. Administration of SARS-CoV Spike protein worsened acute lung failure in mice, and this exacerbation was attenuated by inhibiting the renin-angiotensin pathway.

Why it matters

The study provides in vivo mechanistic evidence that SARS-CoV-mediated ACE2 downregulation drives acute lung failure, suggesting that renin-angiotensin system blockade could serve as a therapeutic strategy.

Limits

All findings are derived from animal models, with no human clinical validation presented. The abstract does not disclose sample sizes, specific control groups, dosing regimens, or quantitative outcome measures.

Cited by