Bogaert · Proceedings of the National Academy of Sciences of the United States of America 2006 · Comparative observational study · n=944

Biological versus nonbiological older brothers and men's sexual orientation.

Cited 237 times in the scientific literature.

Level 4 - case-series / case-control

Comparative observational / case-control study comparing homosexual and heterosexual men

PubMed 16807297 · doi:10.1073/pnas.0511152103 · record verified 2026-08-26

What was done

Four samples of homosexual and heterosexual men (total n = 944), including a cohort raised in nonbiological and blended families (adoptees, step-siblings, or half-siblings), were analyzed to evaluate whether prenatal factors or postnatal/rearing environments account for the fraternal birth-order effect on male sexual orientation.

What was found

The abstract reports no numerical values, odds ratios, or confidence intervals. It states that only the number of biological older brothers predicted men's sexual orientation, whereas nonbiological older brothers and other sibling characteristics showed no predictive association, regardless of time spent reared together.

Why it matters

By differentiating biological from nonbiological siblings and accounting for rearing time, the findings demonstrate that the fraternal birth-order effect is unlikely to be driven by postnatal psychosocial factors, pointing instead toward prenatal biological mechanisms.

Limits

The abstract reports no quantitative effect sizes or statistical test statistics. The design relies on observational and retrospective sibship reports and does not directly measure candidate prenatal biological factors (such as maternal antibodies or hormonal markers).

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