Serafini · The Journal of experimental medicine 2006 · Preclinical animal and in vitro laboratory study · n=?

Phosphodiesterase-5 inhibition augments endogenous antitumor immunity by reducing myeloid-derived suppressor cell function.

Cited 758 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal models and in vitro human cell experiments with no clinical trial data

PubMed 17101732 · doi:10.1084/jem.20061104 · record verified 2026-08-27

What was done

Investigators evaluated phosphodiesterase-5 (PDE5) inhibitors (sildenafil, tadalafil, and vardenafil) as modulators of antitumor immunity across several mouse tumor models and in combination with adoptive T-cell therapy. They also assessed sildenafil's effect on in vitro T-cell proliferation in peripheral blood mononuclear cells isolated from patients with multiple myeloma and head and neck cancer.

What was found

The abstract reports no quantitative values, confidence intervals, or p-values. Qualitatively, PDE5 inhibition delayed tumor progression in mice, downregulated arginase 1 and nitric oxide synthase-2 expression in CD11b+/Gr-1+ myeloid-derived suppressor cells (MDSCs), and enhanced intratumoral T-cell infiltration and activation. Sildenafil improved adoptive T-cell therapy efficacy in mice and restored in vitro proliferation of human patient-derived T cells.

Why it matters

This study identifies a mechanism by which clinically available non-oncology drugs can disrupt tumor-induced immunosuppression by targeting MDSCs. It establishes a preclinical rationale for repurposing PDE5 inhibitors as adjuvants to cancer immunotherapy.

Limits

All findings are preclinical, derived from animal models and in vitro assays rather than human clinical trials. The abstract provides no sample sizes for animal cohorts or human blood donors, and reports no numerical effect sizes, dosing thresholds, or in vivo human clinical outcomes.

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