Hale · The Journal of clinical endocrinology and metabolism 2007 · cross-sectional observational study · n=76

Endocrine features of menstrual cycles in middle and late reproductive age and the menopausal transition classified according to the Staging of Reproductive Aging Workshop (STRAW) staging system.

Cited 190 times in the scientific literature.

Level 4 - case-series / case-control

Cross-sectional observational study characterizing hormonal profiles across four STRAW staging groups over a single cycle.

PubMed 17550960 · doi:10.1210/jc.2007-0066 · record verified 2026-08-29

What was done

Healthy women aged 21–35 and 45–55 submitted blood samples three times per week over a single menstrual cycle. Participants were categorized by STRAW criteria into mid-reproductive age (n = 21), late-reproductive age (n = 16), early menopause transition (n = 16), and late menopause transition (n = 23; total n = 76). Endocrine markers—including FSH, LH, estradiol, luteal progesterone, inhibin B, and anti-Müllerian hormone (AMH)—were measured across ovulatory and anovulatory cycles.

What was found

Anovulatory cycles occurred in 9 women in late menopause transition, 1 in early menopause transition, 0 in late-reproductive age, and 2 in mid-reproductive age. Across ovulatory cycles, advancing STRAW stage was associated with significant increases in FSH (P = 0.001), LH (P < 0.01), and estradiol (P < 0.05), alongside decreased mean luteal-phase progesterone (P < 0.01). Early-cycle inhibin B decreased progressively across STRAW stages (P < 0.01) and was largely undetectable in elongated ovulatory and anovulatory transition cycles. AMH decreased progressively and markedly (10- to 15-fold) across STRAW stages (P < 0.01 and P < 0.001).

Why it matters

The findings show how specific endocrine shifts—notably marked declines in AMH and inhibin B alongside rising gonadotropins—align with clinical STRAW staging criteria for reproductive aging.

Limits

The sample size is modest (76 participants total across four subgroups) and restricted to a single cycle rather than longitudinal follow-up. Absolute hormone concentrations, variability estimates, and confidence intervals are not provided in the abstract.

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