Esnouf · Acupuncture & electro-therapeutics research 2007 · ex vivo laboratory study · n=?

Depth of penetration of an 850nm wavelength low level laser in human skin.

Cited 77 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Ex vivo laboratory measurement on excised human tissue samples.

PubMed 18077939 · doi:10.3727/036012907815844165 · record verified 2026-08-26

What was done

Human abdominal skin samples up to 0.784 mm thick were harvested via dermatome following abdominoplasty procedures. The excised tissue samples were irradiated with a gallium aluminium arsenide laser (850 nm wavelength, 100 mW, 24 kHz, 0.28 mm probe diameter), and transmitted energy was measured directly using an external optical power meter.

What was found

Laser radiation intensity decreased by 66% after passing through a 0.784 mm sample of human abdominal tissue, with the majority of radiation absorbed within the first 1 mm of skin.

Why it matters

These direct tissue measurements provide an empirical estimate of near-infrared laser attenuation in human skin, contrasting with theoretical models that predict penetration depths up to 50 mm.

Limits

The abstract does not state the total number of donors or skin samples evaluated. Measurements were performed ex vivo on non-perfused abdominal skin, which may differ optically from living, vascularized tissue and other anatomical sites. Only a single wavelength, power setting, and maximum thickness (0.784 mm) were tested.

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