Johnstone · The American journal of clinical nutrition 2008 · randomized crossover trial · n=17

Effects of a high-protein ketogenic diet on hunger, appetite, and weight loss in obese men feeding ad libitum.

Cited 443 times in the scientific literature.

Level 2 - randomized trial

Randomized crossover clinical trial

PubMed 18175736 · doi:10.1093/ajcn/87.1.44 · record verified 2026-08-28

What was done

Seventeen obese men participated in a residential, randomized crossover trial comparing two ad libitum, high-protein (30% of energy) diets for 4 weeks each: a low-carbohydrate ketogenic diet (LC; 4% carbohydrate) and a medium-carbohydrate nonketogenic diet (MC; 35% carbohydrate). All food was provided daily. Outcomes included daily body weight, plasma and urinary 3-hydroxybutyrate concentrations, hunger assessed by a computerized visual analogue system, and ad libitum energy intake.

What was found

Ad libitum energy intake was significantly lower on the LC diet compared to the MC diet (7.25 vs. 7.95 MJ/day; P = 0.02; standard error of the difference [SED]: 0.27). Hunger ratings were significantly lower on the LC diet (P = 0.014; SED: 1.76). Over the 4-week period, weight loss was greater on the LC diet than the MC diet (6.34 kg vs. 4.35 kg; P = 0.006; SED: 0.62). The LC diet induced ketosis, with mean 3-hydroxybutyrate concentrations reaching 1.52 mmol/L in plasma (P = 0.036 from baseline; SED: 0.62) and 2.99 mmol/L in urine (P < 0.001 from baseline; SED: 0.36).

Why it matters

This study shows that holding dietary protein constant, restricting carbohydrates sufficiently to induce ketosis provides an additional short-term suppression of hunger and spontaneous energy intake in obese men.

Limits

The trial had a small sample size (n = 17) and enrolled only obese men, limiting generalizability to women and non-obese individuals. The intervention duration was short (4 weeks per arm) in a highly controlled residential setting, leaving long-term adherence, appetite effects, and body composition changes (such as lean mass versus water loss) unmeasured in the abstract.

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