Ward · Archives of internal medicine 2009 · prospective cohort study · n=22169

APOE genotype, lipids, and coronary heart disease risk: a prospective population study.

Cited 84 times in the scientific literature.

Level 3 - non-randomized controlled study

Prospective population-based cohort study

PubMed 19667307 · doi:10.1001/archinternmed.2009.234 · record verified 2026-08-30

What was done

Researchers examined the relationship between APOE genotype (*E3/*E3, *E3/*E4, *E2/*E3, *E4/*E4, *E2/*E4, *E2/*E2) and fatal and nonfatal coronary heart disease (CHD) in 22,169 participants (10,035 men and 12,134 women, aged 40 to 79 years) from the Norfolk, England, arm of the EPIC study. Participants were followed for an average of 11 years (1993-2007) with multivariable adjustments including traditional cardiovascular risk factors and lipid ratios.

What was found

During follow-up, 2,712 CHD events occurred. Compared to homozygous *E3/*E3 individuals, age- and sex-adjusted hazard ratios were 0.88 (95% CI, 0.77-0.99) for *E2 carriers (*E2/*E2 and *E2/*E3) and 1.09 (95% CI, 1.00-1.19) for *E4 carriers (*E3/*E4 and *E4/*E4). Additional adjustment for blood pressure, BMI, diabetes, alcohol, activity, and smoking did not substantially alter findings. However, after further adjustment for baseline ratio of LDL to HDL cholesterol, the hazard ratios attenuated to 0.97 (95% CI, 0.85-1.10) for *E2 carriers and 1.06 (95% CI, 0.97-1.15) for *E4 carriers. No interactions by sex, smoking status, or age groups were observed.

Why it matters

This paper demonstrates in a large cohort that the association between APOE variants and CHD risk is primarily mediated by circulating lipid fractions rather than independent vascular pathways.

Limits

The study is an observational cohort limited to Norfolk, England, which may limit generalizability across diverse racial and ethnic groups. Risk factors and lipid profiles were assessed only at baseline without accounting for subsequent changes or interventions over the 11-year follow-up.

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