Hiraide · Surgery 1991 · controlled clinical trial · n=20

Effect of 3-hydroxybutyrate on posttraumatic metabolism in man.

Cited 25 times in the scientific literature.

Level 3 - non-randomized controlled study

Controlled clinical trial without explicit statement of randomization

PubMed 1992551 · record verified 2026-08-31

What was done

In 20 patients with severe trauma (Injury Severity Score > 20), 11 received an intravenous infusion of DL-3-hydroxybutyrate (3-OHB) at 25 µmol/kg/min for 3 hours, while 9 control patients received sodium DL-lactate at the same rate. Arterial and femoral venous substrate concentrations were measured to assess extremity arteriovenous exchange of ketone bodies, free fatty acids, and amino acids.

What was found

Arterial ketone body increases correlated with increased femoral arteriovenous uptake in the 3-OHB group (R = 0.853, p < 0.001). Compared to controls, venous concentrations and femoral venous-minus-arterial concentration differences of nonesterified free fatty acids, alanine, glycine, and valine decreased significantly with 3-OHB. Specifically, venous alanine concentration fell by 102.3 ± 69.3 µmol/L and the femoral venous-arterial difference decreased by 32.6 ± 22.8 µmol/L (mean ± SD), reflecting suppressed extremity alanine release.

Why it matters

Acute infusion of ketone bodies directly suppresses skeletal muscle amino acid efflux, providing physiological evidence that ketosis may mitigate post-traumatic protein catabolism.

Limits

The study evaluated a very small cohort (n = 20) over a short 3-hour intervention. Randomization and blinding methods are not described in the abstract. Whole-body protein turnover, nitrogen balance, and clinical outcomes were not measured.

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