Meléndez-Hevia · Journal of biosciences 2009 · theoretical metabolic flux analysis · n=?

A weak link in metabolism: the metabolic capacity for glycine biosynthesis does not satisfy the need for collagen synthesis.

Cited 145 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Mechanism-based reasoning and theoretical metabolic flux modeling with no new primary human data.

PubMed 20093739 · doi:10.1007/s12038-009-0100-9 · record verified 2026-08-29

What was done

The authors conducted a theoretical stoichiometric and metabolic flux accounting analysis for an adult human. They compiled published flux estimates for all known pathways of endogenous glycine production and compared total available glycine from de novo synthesis and typical dietary intake against total metabolic demand, particularly for collagen synthesis, in a reference 70 kg human.

What was found

Synthesis from serine accounted for over 85% of total endogenous production, providing approximately 3 g/day. Total available glycine from endogenous synthesis (~3 g/day) plus typical diet (1.5–3.0 g/day) was estimated to fall short of metabolic demand by approximately 10 g/day for a 70 kg human.

Why it matters

This stoichiometric analysis suggests human endogenous synthesis of glycine is constrained and insufficient to meet total metabolic needs, supporting arguments that glycine is a conditionally essential amino acid.

Limits

The study is a theoretical calculation based on secondary literature flux estimates rather than direct human experimental measurements. It did not directly test clinical deficiency states, inter-individual metabolic variability, or in vivo compensatory adaptations.

Cited by