Eisenberg · American journal of physical anthropology 2010 · ecological cross-sectional study · n=?

Worldwide allele frequencies of the human apolipoprotein E gene: climate, local adaptations, and evolutionary history.

Cited 234 times in the scientific literature.

Level 4 - case-series / case-control

Ecological cross-sectional population study (graded by design analogy, not clinical CEBM)

PubMed 20734437 · doi:10.1002/ajpa.21298 · record verified 2026-08-30

What was done

The authors compiled global population allele frequencies for apolipoprotein E (APOE) along with geographic and climatic variables (absolute latitude, elevation, modern temperatures, and estimated Paleolithic temperatures). They tested whether climatic variation predicts APOE epsilon4 distribution and evaluated potential confounding by controlling for population structure in a subset of populations.

What was found

The abstract presents no specific numerical statistics, effect estimates, or sample counts. It describes a curvilinear relationship between APOE epsilon4 allele frequency and absolute latitude, with the lowest frequencies occurring in mid-latitudes. Temperature also showed a curvilinear association with epsilon4 frequency, which improved after accounting for estimated Paleolithic temperatures. Controlling for population structure did not alter the latitude association. In contrast to the authors' hypothesis, elevation did not improve predictive power, and an integrated multi-allele cholesterol score showed a weaker association with latitude than epsilon4 alone.

Why it matters

This study evaluates whether human APOE geographic variation reflects climate-driven metabolic adaptation to thermal extremes rather than neutral demographic history alone, offering mixed observational support for climate-mediated selection.

Limits

The abstract provides no sample sizes (number of populations or individuals analyzed) or quantitative statistical metrics. The study is an ecological association analysis reliant on estimated historical climate reconstructions, and key hypotheses regarding elevation and combined-allele cholesterol effects were not supported.

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