Allyl isothiocyanate-rich mustard seed powder inhibits bladder cancer growth and muscle invasion.
Level 5 - mechanism / opinion, no new human data
Preclinical in vitro and animal model research with no human participants
PubMed 20889681 · doi:10.1093/carcin/bgq202
What was done
Investigators evaluated the anticancer activity of allyl isothiocyanate (AITC)-rich mustard seed powder (MSP-1). In vitro, bladder cancer cell lines were exposed to hydrated MSP-1 to test for apoptosis and cell cycle arrest compared to pure sinigrin with myrosinase. In vivo, rats with orthotopically implanted bladder cancer were treated with oral MSP-1 at 71.5 mg/kg (9 μmol/kg sinigrin dose) to assess tumor growth, muscle invasion, and modulation of key therapeutic targets.
What was found
Hydrated MSP-1 induced apoptosis and G(2)/M phase arrest in bladder cancer cell lines via myrosinase-mediated conversion of sinigrin to AITC. In the orthotopic rat bladder cancer model, oral MSP-1 reduced tumor growth by 34.5% (P < 0.05) and blocked muscle invasion by 100%, alongside significant modulation of vascular endothelial growth factor, cyclin B1, and caspase 3.
Why it matters
The findings demonstrate that mustard seed powder effectively delivers bioavailable AITC to bladder tissue, showing potential as a dietary or botanical agent to inhibit bladder cancer progression.
Limits
The study is limited to cell cultures and a rodent model; human clinical efficacy, optimal dosing, and safety are untested. Exact animal sample sizes and variance metrics are not specified in the abstract.
Cited by
- supports Mustard seed is rich in the glucosinolate sinigrin, which converts upon hydrolysis to allyl isothiocyanate.