Smith · The American journal of clinical nutrition 2011 · randomized controlled trial · n=16

Dietary omega-3 fatty acid supplementation increases the rate of muscle protein synthesis in older adults: a randomized controlled trial.

Cited 662 times in the scientific literature.

Level 2 - randomized trial

Individual randomized controlled trial

PubMed 21159787 · doi:10.3945/ajcn.110.005611 · record verified 2026-08-30

What was done

Sixteen healthy older adults were randomly assigned to receive either dietary omega-3 fatty acids or corn oil (control) for 8 weeks. The rate of muscle protein synthesis and phosphorylation of anabolic signaling elements (mTOR Ser2448 and p70s6k Thr389) were evaluated before and after supplementation under basal postabsorptive conditions and during a hyperaminoacidemic-hyperinsulinemic clamp.

What was found

Corn oil supplementation had no effect on muscle protein synthesis rate or anabolic signaling phosphorylation. Omega-3 supplementation did not change basal muscle protein synthesis (0.051 ± 0.005%/h before vs. 0.053 ± 0.008%/h after; P = 0.80), but significantly augmented the hyperaminoacidemia-hyperinsulinemia-induced increase in muscle protein synthesis rate (from 0.009 ± 0.005%/h above basal to 0.031 ± 0.003%/h above basal; P < 0.01). This was accompanied by increased p70s6k(Thr389) phosphorylation (P < 0.01) and a trend toward increased mTOR(Ser2448) phosphorylation (P = 0.08).

Why it matters

Omega-3 supplementation enhances muscle protein synthesis sensitivity to hyperaminoacidemia and hyperinsulinemia in older adults, offering a potential nutritional strategy against sarcopenia.

Limits

The sample size was very small (n = 16). The trial assessed acute fractional synthesis rates under experimental clamp conditions rather than long-term functional endpoints such as muscle mass or physical strength, and specific supplement dosages were not reported in the abstract.

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