Smuder · Journal of applied physiology (Bethesda, Md. : 1985) 2011 · randomized controlled animal experiment · n=?

Exercise protects against doxorubicin-induced oxidative stress and proteolysis in skeletal muscle.

Cited 137 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Controlled animal experiment (Sprague-Dawley rats)

PubMed 21310889 · doi:10.1152/japplphysiol.00677.2010 · record verified 2026-08-30

What was done

Sprague-Dawley rats were randomly assigned to four groups: sedentary, exercise, sedentary with doxorubicin (Dox), or exercise with Dox. The study evaluated whether exercise training attenuated Dox-induced skeletal muscle oxidative stress, activation of cellular proteases (calpain and caspase-3), and alterations in cytoprotective proteins including antioxidant enzymes and heat shock protein 72.

What was found

The abstract reports no numerical data or statistical effect sizes. Doxorubicin increased oxidative stress and activated calpain and caspase-3 in rat skeletal muscle. Prior exercise training prevented Dox-induced oxidative damage and protease activation, which coincided with increased muscle levels of antioxidant enzymes and heat shock protein 72.

Why it matters

This study indicates that exercise training can counteract doxorubicin-induced myotoxicity, providing a mechanistic rationale for exercise interventions during chemotherapy.

Limits

This was an animal study conducted in rats, limiting direct clinical translation to humans receiving cancer chemotherapy. The abstract provides no sample size, no specific drug dosages or exercise protocol parameters, and no numerical values or functional muscle performance outcomes.

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