Little · Journal of applied physiology (Bethesda, Md. : 1985) 2011 · single-arm pre-post interventional study · n=8

Low-volume high-intensity interval training reduces hyperglycemia and increases muscle mitochondrial capacity in patients with type 2 diabetes.

Cited 792 times in the scientific literature.

Level 4 - case-series / case-control

Uncontrolled single-arm pre-post interventional study

PubMed 21868679 · doi:10.1152/japplphysiol.00921.2011 · record verified 2026-08-30

What was done

Eight patients with type 2 diabetes (mean age 63 ± 8 years, BMI 32 ± 6 kg/m², HbA1c 6.9 ± 0.7%) completed a 2-week intervention consisting of six sessions of low-volume high-intensity interval training (10 × 60-second cycling intervals at ~90% maximal heart rate with 60-second rest intervals). Glucose control was measured before and 48–72 hours after the final exercise session using 24-hour continuous glucose monitoring under standardized dietary conditions. Skeletal muscle biopsy samples from the vastus lateralis were analyzed for mitochondrial enzyme activity and key metabolic proteins.

What was found

Average 24-hour blood glucose decreased from 7.6 ± 1.0 mmol/l before training to 6.6 ± 0.7 mmol/l after training (P < 0.05). Postprandial glucose area under the curve across breakfast, lunch, and dinner was significantly reduced (P < 0.05). In skeletal muscle, citrate synthase maximal activity increased by ~20% (P < 0.05), and protein contents of Complex II 70 kDa (~37%), Complex III Core 2 (~51%), Complex IV subunit IV (~68%), Mitofusin 2 (~71%), and GLUT4 (~369%) all significantly increased (all P < 0.05).

Why it matters

This study provides early proof-of-concept that very low-volume interval training can rapidly lower daily glycemic exposure and upregulate skeletal muscle oxidative machinery in patients with type 2 diabetes.

Limits

The study is an uncontrolled, single-arm pre-post trial with a very small sample size (n = 8). The intervention lasted only 2 weeks, leaving long-term adherence, durability of adaptations, and clinical safety unaddressed.

Cited by