Hughes · Journal of agricultural and food chemistry 2011 · Inter-laboratory analytical comparative study · n=4 soy ingredients

Protein digestibility-corrected amino acid scores (PDCAAS) for soy protein isolates and concentrate: criteria for evaluation.

Cited 313 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Bench/laboratory analytical comparison study without human clinical data (Level 5 by CEBM design analogy)

PubMed 22017752 · doi:10.1021/jf203220v · record verified 2026-08-27

What was done

PDCAAS (protein digestibility-corrected amino acid score) values were evaluated for four processed soy ingredients—three isolated soy proteins (ISP) and one soy protein concentrate (SPC). Amino acid profiling and true digestibility measurements were compared across two independent laboratories to assess the impact of analytical method accuracy and reproducibility.

What was found

Amino acid values from the first laboratory produced a truncated PDCAAS score of 1.00 for all four ingredients. The second laboratory yielded statistically significantly lower PDCAAS scores, ranging from 0.95 to 1.00. The authors found that analytical method error substantially contributes to these discrepancies and can be mitigated by applying amino acid nitrogen recovery correction factors.

Why it matters

It shows that published differences in soy protein quality scores can result from laboratory measurement artifacts rather than intrinsic ingredient differences, supporting standard nitrogen recovery corrections.

Limits

The analysis was restricted to four soy ingredients tested at only two laboratories. The abstract reports no human in vivo data, raw amino acid recovery metrics, or exact confidence intervals.

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