Macfarlane · Journal of AOAC International 2012 · narrative review · n=?

Bacteria, colonic fermentation, and gastrointestinal health.

Cited 964 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Narrative review describing biological mechanisms without original clinical data or systematic methodology.

PubMed 22468341 · doi:10.5740/jaoacint.sge_macfarlane · record verified 2026-08-29

What was done

This is a narrative review summarizing the physiological roles of the colonic microbiome in digestive physiology, focusing on the catabolism of carbohydrates and proteins and their associated metabolic end products.

What was found

The abstract provides no quantitative data or specific numbers. It describes qualitatively that carbohydrate and protein digestion generate short-chain fatty acids (acetate, propionate, butyrate), while protein catabolism also yields diverse potentially toxic end products such as amines, phenols, indoles, thiols, and hydrogen sulfide. It notes that depletion of carbohydrates along the colon may correspond to higher rates of disease in the distal bowel.

Why it matters

It conceptualizes the balance between saccharolytic and proteolytic fermentation across the large intestine, highlighting how nutrient availability influences mucosal exposure to toxic metabolites.

Limits

The paper is a narrative overview without systematic search methods, clinical trials, or quantitative effect estimates. It relies on generalized physiological models rather than empirical intervention data.

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