Abdelwahab · PloS one 2012 · Animal experimental study · n=?

The ketogenic diet is an effective adjuvant to radiation therapy for the treatment of malignant glioma.

Cited 288 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal model study without human participants

PubMed 22563484 · doi:10.1371/journal.pone.0036197 · record verified 2026-08-30

What was done

Mice with intracranial bioluminescent malignant glioma implants were assigned to either a standard diet or a 4:1 ketogenic formula (KetoCal). Mice received 2 x 4 Gy whole-brain radiation, and tumor growth was monitored by in vivo imaging. Animals in the combined treatment group were returned to a standard diet at day 101 post-implantation to monitor for tumor recurrence.

What was found

Animals fed the ketogenic formula had significantly elevated beta-hydroxybutyrate levels (p = 0.0173) and an increased median survival of approximately 5 days compared to standard diet controls. Combining the ketogenic diet with radiation produced a greater-than-additive effect, reducing tumor bioluminescence below the detection threshold in 9 of 11 animals (p < 0.0001), with no recurrence observed for over 200 days following return to a standard diet.

Why it matters

This study provides preclinical proof-of-concept that dietary ketosis may act as a radiosensitizer in malignant glioma, offering a mechanistic rationale for testing ketogenic adjuvants in human neuro-oncology trials.

Limits

The study was conducted entirely in a rodent model, so clinical efficacy, adherence, and safety in human patients remain unknown. The total sample size across all treatment arms is not reported in the abstract.

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