Hatori · Cell metabolism 2012 · controlled animal laboratory experiment · n=?

Time-restricted feeding without reducing caloric intake prevents metabolic diseases in mice fed a high-fat diet.

Cited 1999 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal research with no human data (Oxford CEBM Level 5)

PubMed 22608008 · doi:10.1016/j.cmet.2012.04.019 · record verified 2026-08-30

What was done

Mice were fed an isocaloric high-fat diet (HFD) under either ad libitum conditions or an 8-hour daily time-restricted feeding (tRF) schedule to determine whether metabolic disease is driven by the diet itself or by disruption of feeding and metabolic cycles. Outcomes evaluated included body weight, hyperinsulinemia, hepatic steatosis, inflammation, motor coordination, signaling pathways (CREB, mTOR, AMPK), circadian clock gene oscillations, liver metabolome, and energy expenditure.

What was found

The abstract reports no numerical values, effect sizes, or confidence intervals. Directionally, mice in the 8-hour tRF group consumed equivalent calories from the high-fat diet as ad libitum controls but were protected against obesity, hyperinsulinemia, hepatic steatosis, and inflammation, alongside improved motor coordination, enhanced CREB, mTOR, and AMPK pathway function, preserved circadian clock oscillations, altered liver metabolome, and improved nutrient utilization and energy expenditure.

Why it matters

This study shows in a rodent model that restricting the daily feeding window can prevent diet-induced obesity and metabolic pathology without reducing total caloric intake.

Limits

The study was conducted exclusively in mice and findings cannot be directly applied to humans without clinical trials. The abstract omits sample sizes (n), study duration, and all quantitative measurements and statistical effect sizes.

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