· Nature 2012 · Cross-sectional cohort study · n=?

Structure, function and diversity of the healthy human microbiome.

Cited 12118 times in the scientific literature.

Level 4 - case-series / case-control

Cross-sectional observational profiling study (design analogy)

PubMed 22699609 · doi:10.1038/nature11234 · record verified 2026-08-26

What was done

As part of the Human Microbiome Project, researchers analyzed microbial communities across multiple distinct, clinically relevant body habitats (including gut, skin, and vagina) in a cohort of healthy human subjects to characterize taxonomic diversity, niche specialization, and metagenomic metabolic pathway carriage.

What was found

Microbial diversity and abundance varied widely across healthy subjects with strong niche specialization within and between individuals. The study captured an estimated 81-99% of the genera, enzyme families, and community configurations typical of the healthy Western microbiome. Metagenomic carriage of metabolic pathways was stable among individuals despite community structure variation, and ethnic/racial background showed one of the strongest associations with both pathways and microbes. The abstract reports no specific participant count or test statistics.

Why it matters

This work provides a foundational reference catalog of baseline structural and functional configurations of the healthy human microbiome, establishing a benchmark for disease and translational research.

Limits

The abstract omits exact sample sizes (n) and demographic details. Findings represent an observational snapshot of healthy Western individuals, limiting generalizability to non-Western populations or diseased cohorts, and do not establish causal mechanisms behind observed variations.

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