Navratilova · Proceedings of the National Academy of Sciences of the United States of America 2012 · Preclinical animal experiment · n=?

Pain relief produces negative reinforcement through activation of mesolimbic reward-valuation circuitry.

Cited 320 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal laboratory experiment with no human clinical data.

PubMed 23184995 · doi:10.1073/pnas.1214605109 · record verified 2026-08-29

What was done

In an experimental postsurgical pain animal model, researchers tested whether blocking injury-related afferent input with a local anesthetic produces conditioned place preference. They measured activation of ventral tegmental dopaminergic cells and dopamine release in the nucleus accumbens, and assessed whether conditioned place preference could be blocked by injecting dopamine antagonists into the nucleus accumbens.

What was found

The abstract reports no numerical values, effect sizes, or statistical metrics. Blockade of afferent input from injury elicited conditioned place preference, activated ventral tegmental dopaminergic neurons, and increased dopamine release in the nucleus accumbens. The conditioned place preference was blocked by dopamine antagonists injected into the nucleus accumbens.

Why it matters

This study provides direct mechanistic evidence that pain relief functions as a negative reinforcer by activating canonical mesolimbic dopamine reward-valuation circuitry.

Limits

The abstract does not report the animal species, sample sizes, or quantitative data. As a preclinical animal model of acute postsurgical pain, the findings may not directly translate to human pain processing or chronic pain conditions.

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