Touitou · European journal of applied physiology and occupational physiology 1990 · observational comparative study · n=4

Effect of shift work on the night-time secretory patterns of melatonin, prolactin, cortisol and testosterone.

Cited 149 times in the scientific literature.

Level 4 - case-series / case-control

Small observational cross-sectional study with non-randomized control comparison

PubMed 2357985 · doi:10.1007/BF00379398 · record verified 2026-08-28

What was done

Researchers evaluated nocturnal hormone secretion in 4 male oil refinery operators aged 25-34 years on a fast-rotating shift schedule (every 3-4 days), drawn from a larger study of 12 shift workers. Blood was sampled every 2 hours during a night shift (0000 to 0800 hours) to measure secretory profiles of melatonin, prolactin, cortisol, and testosterone compared to controls.

What was found

Melatonin peak values were higher in shift workers, resulting in a four-times higher rhythm amplitude than in controls. Prolactin and testosterone concentrations were significantly decreased, with erratic peak and trough times. Serum cortisol showed decreased rhythm amplitude with higher concentrations at 0000 hours in shift workers. No apparent phase shift was observed for any hormone. The abstract provides relative changes but omits exact numerical concentrations and variance.

Why it matters

The findings indicate that fast-rotating shift work alters hormone levels and amplitudes during night shifts without necessarily causing circadian phase shifts.

Limits

The sample size of sampled shift workers was extremely small (n = 4), and details regarding the control group are absent. Sampling was restricted to an 8-hour nighttime window rather than a complete 24-hour cycle, and absolute numerical values and statistical test metrics were not reported in the abstract.

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