Breen · The Journal of clinical endocrinology and metabolism 2013 · prospective pre-post study · n=10

Two weeks of reduced activity decreases leg lean mass and induces "anabolic resistance" of myofibrillar protein synthesis in healthy elderly.

Cited 392 times in the scientific literature.

Level 4 - case-series / case-control

Single-arm prospective pre-post intervention study without a separate control group

PubMed 23589526 · doi:10.1210/jc.2013-1502 · record verified 2026-08-31

What was done

Ten healthy older adults (age 72 ± 1 y) underwent 14 days of reduced daily step count. Before and after the intervention, body composition, physical function, muscle strength, and insulin sensitivity were measured. Fasted, postabsorptive, and postprandial myofibrillar protein synthesis (MPS) rates were quantified using primed constant infusions of L-[ring-(13)C6]phenylalanine with serial muscle biopsies.

What was found

Daily step count decreased by ~76% to 1413 ± 110 steps/day. This intervention resulted in: - Leg fat-free mass reduction of ~3.9% (P < .001). - Postabsorptive insulin resistance increase of ~12% and postprandial insulin sensitivity reduction of ~43% (P < .005). - Increases in TNF-α (~12%) and C-reactive protein (~25%) (P < .05). - Postprandial MPS reduction of ~26% (P = .028), with no change in postabsorptive MPS rates.

Why it matters

It demonstrates that brief periods of reduced daily ambulation induce anabolic resistance and rapid muscle loss in healthy elderly adults, alongside metabolic and inflammatory declines.

Limits

The study is limited by a very small sample size (n = 10) and an uncontrolled, single-arm design. Long-term recovery or reversibility after resuming baseline activity was not reported in the abstract.

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