Obermann-Borst · Pediatric research 2013 · Observational cohort study · n=?

Duration of breastfeeding and gender are associated with methylation of the LEPTIN gene in very young children.

Cited 110 times in the scientific literature.

Level 3 - non-randomized controlled study

Observational follow-up study examining associations between early life factors and gene methylation at 17 months

PubMed 23884163 · doi:10.1038/pr.2013.95 · record verified 2026-08-26

What was done

The authors examined associations between maternal education, breastfeeding duration, and child constitutional factors (sex, birth weight, BMI at 17 months) with DNA methylation of the leptin (LEP) gene in whole blood and serum leptin concentration at 17 months of age.

What was found

Duration of breastfeeding was negatively associated with LEP methylation. Low maternal education (≤12 years) was associated with higher LEP methylation. Boys had higher birth weight and lower LEP methylation than girls. Birth weight per SD increase (+584 g) was inversely associated with LEP methylation. Higher BMI and higher serum leptin concentrations were also associated with lower LEP methylation. Specific numerical estimates, effect sizes, confidence intervals, and p-values were not reported in the abstract.

Why it matters

This study provides preliminary observational evidence linking early nutritional and environmental exposures to epigenetic variation in a gene central to energy homeostasis and appetite regulation.

Limits

The abstract does not state the sample size (n), effect sizes, or confidence intervals. The observational design cannot establish causality between infant feeding practices and DNA methylation. DNA methylation was measured in whole blood rather than target metabolic tissues, and functional consequences or clinical outcomes were not assessed.

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