Poutahidis · PloS one 2013 · controlled animal experiment · n=?

Microbial symbionts accelerate wound healing via the neuropeptide hormone oxytocin.

Cited 310 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal and mechanistic laboratory research

PubMed 24205344 · doi:10.1371/journal.pone.0078898 · record verified 2026-08-28

What was done

Researchers administered lactic acid bacteria (specifically Lactobacillus reuteri) via drinking water to animals and compared wound-healing rates against matched controls. They evaluated the mechanistic pathway by assessing vagus nerve involvement, systemic oxytocin upregulation, and the role of activated CD4+Foxp3+CD25+ regulatory T cells via adoptive transfer into naive Rag2-deficient animals.

What was found

Probiotic supplementation accelerated the wound-healing process to half the time required for matched control animals. Lactobacillus reuteri upregulated oxytocin via a vagus nerve-dependent pathway, driving the activation of CD4+Foxp3+CD25+ regulatory T cells that successfully transferred accelerated wound healing to naive Rag2-deficient animals. Exact animal sample sizes, absolute healing durations in days, and variance metrics were not provided in the abstract.

Why it matters

The findings identify a gut-brain-immune axis where specific commensal gut microbes stimulate neuroendocrine oxytocin signaling to enhance tissue repair through regulatory T cells. This mechanistic pathway links dietary probiotics directly to peripheral wound healing in an animal model.

Limits

The study was conducted entirely in animal models, preventing direct generalization to humans. The abstract does not disclose exact sample sizes, animal species or strains (aside from Rag2-deficient recipients), quantitative rates of healing, or statistical significance metrics.

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