Dai · Journal of food science 2014 · In vitro laboratory experiment · n=?

Release of allyl isothiocyanate from mustard seed meal powder.

Cited 66 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Bench laboratory experiment evaluating chemical release kinetics with no human or in vivo data.

PubMed 24313968 · doi:10.1111/1750-3841.12322 · record verified 2026-08-30

What was done

Defatted and partially defatted mustard seed meals were ground into powders with particle sizes from 5 to 300 μm. Samples were sealed in glass jars adjusted to 85% or 100% relative humidity at 5, 20, or 35 °C. The release of volatile allyl isothiocyanate (AITC) was quantified using gas chromatography.

What was found

AITC release rate and total amount increased with higher relative humidity and higher temperature. Release rates were also influenced by particle size and lipid content. Across the tested conditions, AITC release ranged from 2 to 17 mg/g of mustard seed meal powder within 24 hours.

Why it matters

It demonstrates that ambient humidity can activate the enzymatic release of antimicrobial AITC from mustard byproduct meal, supporting its potential development as a natural active packaging material.

Limits

This was an in vitro chemical release study with no direct tests on microbial growth, food shelf life, or sensory impacts on food products. Precise sample sizes and statistical variance measures were not reported in the abstract.

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