Layton · The Journal of clinical endocrinology and metabolism 2014 · retrospective incident user cohort study · n=1597346

Testosterone lab testing and initiation in the United Kingdom and the United States, 2000 to 2011.

Cited 196 times in the scientific literature.

Level 3 - non-randomized controlled study

Retrospective observational cohort study using claims and electronic health records

PubMed 24423353 · doi:10.1210/jc.2013-3570 · record verified 2026-08-27

What was done

Researchers performed a retrospective incident user cohort study analyzing US commercial and Medicare claims and UK general practitioner electronic health records from 2000 to 2011. They evaluated 410,019 US men and 6,858 UK men initiating testosterone formulations (injections, implants, transdermal, or oral), alongside 1,114,329 US men and 66,140 UK men undergoing a new testosterone laboratory measurement.

What was found

Testosterone testing and supplementation increased substantially over the decade, especially in the US. UK testing primarily identified men with low testosterone, whereas US testing increasingly captured men with normal levels. Men in the US were more likely to start treatment despite having normal testosterone levels, and a substantial proportion initiated therapy without recent laboratory testing. Transdermal gels emerged as the predominant initial formulation in both countries. Exact numerical rates and proportions were not reported in the abstract.

Why it matters

This study documents widespread international increases in testosterone therapy and highlights major practice gaps, particularly in the US where treatment was frequently initiated without documented biochemical deficiency or preceding lab tests.

Limits

The abstract reports no exact percentages, effect sizes, or laboratory threshold values. The study relies on administrative claims and primary care records, which may miss unbilled care, cash purchases, or specific clinical indications.

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