Cisternas · Journal of neurochemistry 2014 · In vitro experimental study · n=?

The oxidized form of vitamin C, dehydroascorbic acid, regulates neuronal energy metabolism.

Cited 73 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

In vitro laboratory study in rodent cell culture

PubMed 24460956 · doi:10.1111/jnc.12663 · record verified 2026-08-30

What was done

Primary cultures of rat brain cortical neurons were used to evaluate the cellular uptake of ascorbic acid (AA) and its oxidized form, dehydroascorbic acid (DHA). Researchers tracked intracellular AA oxidation, changes in reduced glutathione levels, glucose flux through glycolysis versus the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase activity, and neuronal lactate uptake.

What was found

Rat cortical neurons took up both AA and DHA, with intracellular AA rapidly oxidizing to DHA. DHA exposure caused a rapid, transient decline in reduced glutathione followed by recovery. This was accompanied by increased glucose oxidation through the PPP, stimulation of glucose-6-phosphate dehydrogenase activity, and a decrease in glycolytic glucose oxidation. Additionally, DHA stimulated neuronal lactate uptake in a time- and dose-dependent manner. The abstract reported no specific numerical values or statistical metrics.

Why it matters

This study outlines a mechanistic role for oxidized vitamin C (DHA) in regulating brain bioenergetics, suggesting it shifts neuronal glucose utilization toward antioxidant defense via the pentose phosphate pathway while promoting lactate utilization.

Limits

The findings are derived entirely from in vitro primary rodent neuronal cultures, limiting direct extrapolation to intact mammalian or human brains. The abstract does not provide sample sizes, replicate numbers, or quantitative effect sizes.

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