Cheng · Cell stem cell 2014 · Animal experiment and mechanistic study · n=?

Prolonged fasting reduces IGF-1/PKA to promote hematopoietic-stem-cell-based regeneration and reverse immunosuppression.

Cited 500 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal and mechanistic study with preliminary human clinical observation

PubMed 24905167 · doi:10.1016/j.stem.2014.04.014 · record verified 2026-08-30

What was done

Researchers investigated how cycles of prolonged fasting affect long-term hematopoietic stem cells (LT-HSCs), niche cells, and immune system regeneration. The study examined circulating IGF-1 levels, PKA activity, stress resistance, self-renewal, and lineage balance in mice subjected to fasting, chemotherapy, or aging, supplemented with mechanistic manipulations of IGF-1 and PKA pathways and preliminary observations in fasting chemotherapy patients.

What was found

The abstract reports no numerical values. Prolonged fasting reduced circulating IGF-1 levels and PKA activity across various cell populations. In mice, multiple cycles of fasting lowered chemotherapy-induced immunosuppression and mortality and reversed age-dependent myeloid bias. These regenerative effects were mimicked by IGF-1 or PKA deficiency and blocked by exogenous IGF-1. Preliminary observations indicated lymphocyte protection from chemotoxicity in fasting patients.

Why it matters

The study identifies a dietary signaling axis (IGF-1/PKA downregulation via prolonged fasting) that promotes stem-cell-driven hematopoietic regeneration and protects against chemotherapy toxicity in preclinical models.

Limits

The abstract contains no quantitative data, sample sizes, or effect sizes. The primary findings are restricted to mouse models, and the human data are described only as preliminary with unspecified study design, sample size, and clinical endpoints.

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