Shukla · Cancer & metabolism 2014 · Preclinical in vitro and animal experiment · n=?

Metabolic reprogramming induced by ketone bodies diminishes pancreatic cancer cachexia.

Cited 257 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical cell line and animal research without human participants.

PubMed 25228990 · doi:10.1186/2049-3002-2-18 · record verified 2026-08-30

What was done

Researchers investigated the effects of ketone bodies and a ketogenic diet on tumor metabolism and cancer-induced cachexia. In vitro experiments using multiple pancreatic cancer cell lines assessed glycolytic flux, glutamine uptake, ATP content, apoptosis, and c-Myc regulation and promoter binding. In vivo effects on tumor growth, muscle loss, and body weight were tested using mouse orthotopic xenograft models fed a ketogenic diet.

What was found

No quantitative numbers, effect sizes, or p-values were reported in the abstract. Directionally, ketone body treatment decreased glycolytic flux, glutamine uptake, ATP levels, and cell survival while inducing apoptosis in pancreatic cancer cell lines. It also reduced c-Myc levels and its recruitment to glycolytic gene promoters. In cell models and mouse orthotopic xenografts, ketone bodies and a ketogenic diet diminished cachexia, slowed tumor growth, and inhibited muscle and body weight loss.

Why it matters

This work identifies a metabolic mechanism through which ketone bodies may downregulate c-Myc and glycolytic pathways, suggesting ketogenic dietary strategies could potentially counter pancreatic cancer cachexia.

Limits

The study is restricted to in vitro cell lines and mouse xenografts; no human clinical data were collected. The abstract omits sample sizes, quantitative measurements, specific dietary macronutrient compositions, and statistical uncertainty. Rodent models of cachexia and metabolism often do not directly translate to human disease.

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