Suez · Nature 2014 · Controlled animal and translational mechanistic study · n=?

Artificial sweeteners induce glucose intolerance by altering the gut microbiota.

Cited 2060 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Animal model and mechanistic laboratory study with preliminary human translational experiments

PubMed 25231862 · doi:10.1038/nature13793 · record verified 2026-08-30

What was done

The authors assessed the impact of non-caloric artificial sweeteners (NAS) on glucose tolerance and the intestinal microbiome in mice and healthy humans. They evaluated the effects of antibiotic treatment on NAS-induced metabolic changes and performed fecal microbiota transplants into germ-free mice using stool from NAS-consuming mice or cultures anaerobically incubated with NAS.

What was found

The abstract reports no numerical values, effect sizes, or participant numbers. Qualitatively, NAS consumption induced gut dysbiosis and glucose intolerance. These metabolic effects were abolished by antibiotics and transferred to germ-free mice via fecal transplantation. Similar dysbiosis and glucose intolerance were observed in healthy humans.

Why it matters

This study provides evidence of a biological mechanism whereby artificial sweeteners may alter gut microbial ecology and impair glucose regulation, challenging assumptions about their metabolic neutrality.

Limits

The abstract provides no sample sizes, specific sweetener types, dosages, durations of intervention, or quantitative data. The primary causal mechanisms rely on rodent and in vitro models, and details regarding the human experimental design are omitted.

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