Irmak · Sleep 2014 · Animal optogenetic interventional study · n=?

Basal forebrain cholinergic modulation of sleep transitions.

Cited 136 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Preclinical animal research (optogenetics in mice)

PubMed 25325504 · doi:10.5665/sleep.4246 · record verified 2026-08-26

What was done

Combined optogenetic photostimulation of cholinergic neurons in the substantia innominata (basal forebrain) with surface EEG recordings in freely moving, naturally sleeping mice to investigate state transitions from NREM sleep to REM sleep or wakefulness.

What was found

The abstract reports no numerical values, exact latencies, or statistical metrics. Photo-stimulation of basal forebrain cholinergic neurons during NREM sleep elicited cortical activation and facilitated transitions to wakefulness and arousal on a time scale similar to other subcortical arousal systems.

Why it matters

Provides causal, cell-type-specific evidence in rodents demonstrating that basal forebrain cholinergic neuron activation is sufficient to trigger cortical desynchronization and transitions from NREM sleep to wakefulness.

Limits

Preclinical rodent model with unclear quantitative generalizability to human physiology. The abstract provides no sample size (n), quantitative data, effect sizes, or control-group metrics, and it does not report loss-of-function (inhibition) findings.

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