Tourani · Plastic and reconstructive surgery 2014 · cadaveric anatomical study · n=15 cadavers (32 limbs)

Understanding the three-dimensional anatomy of the superficial lymphatics of the limbs.

Cited 48 times in the scientific literature.

Level 5 - mechanism / opinion, no new human data

Human cadaveric anatomical and histological study (mechanism/bench/ex vivo)

PubMed 25347638 · doi:10.1097/PRS.0000000000000640 · record verified 2026-08-26

What was done

Injection, microdissection, radiographic, and histologic examinations were performed on the superficial lymphatics and subcutaneous tissue layers of 32 limbs from 15 human cadavers.

What was found

Five consistent tissue layers were identified across all specimens: (1) skin, (2) subcutaneous fat, (3) superficial fascia, (4) loose areolar tissue, and (5) deep fascia. Subcutaneous fat was subdivided by a horizontal septum (layer 2b) into superficial (2a) and deep (2c) compartments. Main superficial veins and nerves ran in layer 4, while lymphatic collectors were located specifically in layers 2c and 4. The abstract provides no quantitative dimensions or measurements.

Why it matters

Defining the anatomical layers and depth of superficial lymphatic collectors helps guide lymphatic surgery, such as lymphovenous anastomosis, and informs surgical planes to avoid iatrogenic injury during body-contouring procedures.

Limits

Findings are based on a small sample of 15 non-living cadaver specimens, which may not reflect living physiology or altered tissue planes in chronic lymphedema. The abstract reports no quantitative measurements, vessel diameters, or demographic characteristics.

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