Mohajeri · The British journal of nutrition 2015 · randomized placebo-controlled trial · n=59

Chronic treatment with a tryptophan-rich protein hydrolysate improves emotional processing, mental energy levels and reaction time in middle-aged women.

Cited 80 times in the scientific literature.

Level 2 - randomized trial

Randomized, placebo-controlled, parallel-group clinical trial

PubMed 25572038 · doi:10.1017/S0007114514003754 · record verified 2026-08-29

What was done

Fifty-nine healthy women aged 45–65 were randomized to receive either a tryptophan-rich egg protein hydrolysate supplement (n = 29) or a placebo (n = 30) at a dose of 0.5 g twice daily for 19 days in a parallel-group trial. Cognitive function, emotional processing (evaluated via an Affective Go/No-Go Task and a Facial Emotional Expression Rating Task), mood state, and sleep quality were measured.

What was found

The abstract reports no exact numerical values, effect sizes, or p-values. It reports that tryptophan supplementation significantly shifted emotional processing away from negative stimuli, slowed responses to negative words on the Affective Go/No-Go Task, and biased facial expression ratings toward happiness. Treated participants also experienced an increase in arousal-like symptoms ('high energy'), shorter reaction times, a slight benefit to sustained attention, and a self-reported feeling of happiness before bedtime when taken 60–90 minutes before sleep.

Why it matters

Provides preliminary clinical trial evidence that low-dose dietary tryptophan from protein hydrolysate can modulate cognitive and emotional processing in healthy middle-aged individuals without pharmaceutical intervention.

Limits

The abstract reports directional findings only, without providing effect sizes, variance, or p-values. The sample size was modest (59 participants) and limited exclusively to healthy middle-aged women, precluding direct generalization to men, other age groups, or clinical populations with mood disorders. Treatment duration was brief (19 days).

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